Genetic engineering approach for the production of rhamnosyl and allosyl flavonoids from Escherichia coli.

@article{Simkhada2010GeneticEA,
  title={Genetic engineering approach for the production of rhamnosyl and allosyl flavonoids from Escherichia coli.},
  author={Dinesh Simkhada and Hei Chan Lee and Jae Kyung Sohng},
  journal={Biotechnology and bioengineering},
  year={2010},
  volume={107 1},
  pages={
          154-62
        }
}
The main functions of glycosylation are stabilization, detoxification and solubilization of substrates and products. To produce glycosylated products, Escherichia coli was engineered by overexpression of TDP-L-rhamnose and TDP-6-deoxy-D-allose biosynthetic gene clusters, and flavonoids were glycosylated by the overexpression of the glycosyltransferase gene from Arabidopsis thaliana. For the glycosylation, these flavonoids (quercetin and kaempferol) were exogenously fed to the host in a… CONTINUE READING
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