Genetic effects of oxidative DNA damages: comparative mutagenesis of the imidazole ring-opened formamidopyrimidines (Fapy lesions) and 8-oxo-purines in simian kidney cells

  title={Genetic effects of oxidative DNA damages: comparative mutagenesis of the imidazole ring-opened formamidopyrimidines (Fapy lesions) and 8-oxo-purines in simian kidney cells},
  author={M. Abul Kalam and Kazuhiro Haraguchi and Sushil Chandani and Edward L. Loechler and Maasaki Moriya and Marc M. Greenberg and Ashis K Basu},
  journal={Nucleic Acids Research},
  pages={2845 - 2845}
Fapy·dG and 8-oxo-7,8-dihydro-2 0-deoxyguanosine (8-oxo-dG) are formed in DNA by hydroxyl radical damage. In order to study replication past these les-ions in cells, we constructed a single-stranded shuttle vector containing the lesion in 5 0-TGT and 5 0-TGA sequence contexts. Replication of the modified vector in simian kidney (COS-7) cells showed that Fapy·dG is mutagenic inducing primarily targeted Fapy·G!T transversions. In the 5 0-TGT sequence mutational frequency of Fapy·dG was $30… Expand

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