Genetic diversity within Lactobacillus sakei and Lactobacillus curvatus and design of PCR primers for its detection using randomly amplified polymorphic DNA.

@article{Berthier1999GeneticDW,
  title={Genetic diversity within Lactobacillus sakei and Lactobacillus curvatus and design of PCR primers for its detection using randomly amplified polymorphic DNA.},
  author={Françoise Berthier and Stanislav Dusko Ehrlich},
  journal={International journal of systematic bacteriology},
  year={1999},
  volume={49 Pt 3},
  pages={
          997-1007
        }
}
  • F. Berthier, S. Ehrlich
  • Published 1 July 1999
  • Biology, Medicine
  • International journal of systematic bacteriology
The genotypic and phenotypic diversity among isolates of the Lactobacillus curvatus/Lactobacillus graminis/Lactobacillus sakei group was evaluated by comparing RAPD data and results of biochemical tests, such as hydrolysis of arginine, D-lactate production, melibiose and xylose fermentation, and the presence of haem-dependent catalase. Analyses were applied to five type strains and to a collection of 165 isolates previously assigned to L. sakei or L. curvatus. Phenotypic and RAPD data were… 
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These molecular methods offer a fast and economic way for the identification and characterization of lactic acid bacterial with a potential use as starter cultures in the elaboration of handmade fermented products.
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TLDR
The results obtained indicate that strain CCUG 34545T and its duplicate, CCUG 41580T, are Lactobacillus sakei subsp.
Characterization of Lactobacillus sakei by the type of stereoisomers of lactic acid produced.
TLDR
The change of the type of stereoisomers of lactic acid from the DL-type to the L-type in the presence of sodium acetate was concluded to be species-specific for L. sakei and useful for identification of strains in this species.
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The results suggest that, for various well-preserved silage preparations, the isolates may be useful in producing novel inoculants corresponding to their optimally climatic and ecological niches.
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References

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TLDR
A numerical analysis of total soluble cell protein patterns and biochemical test data revealed that there are two phenotypic sub groups within L, curvatus and two phenotypesic subgroups within L. sake, and it is proposed that strains of L. bavaricus are reclassified as members ofL.
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TLDR
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TLDR
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The taxonomic relationships of six strains of Lactobacillus bavaricus, including type strain 136 (= DSM 20269) and reference strain Tro-8/78, Lactobacillus curvatus, and Lactobacillus sake were…
Rapid species identi ¢ cation within two groups of closely related lactobacilli using PCR primers that target the 16 S / 23 S rRNA spacer region Franc °
TLDR
A rapid and reliable PCR-based method for distinguishing closely related species within two groups of lactobacilli is described, which allowed the same species identification as the DNA/DNA hybridization procedure.
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Genetic relationships among strains of Xanthomonas fragariae based on random amplified polymorphic DNA PCR, repetitive extragenic palindromic PCR, and enterobacterial repetitive intergenic consensus PCR data and generation of multiplexed PCR primers useful for the identification of this phytopathoge
TLDR
There was no correlation between DNA amplification product patterns and geographic sites of isolation, suggesting that this bacterium has spread largely through exchange of infected plant germ plasm.
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