Genetic diversity of Babesia bovis in virulent and attenuated strains.

  title={Genetic diversity of Babesia bovis in virulent and attenuated strains.},
  author={Monica L. Mazuz and Thea Molad and Lea Fish and Benjamin Leibovitz and Ricardo Wolkomirsky and Ludmila Fleiderovitz and Varda Shkap},
  volume={139 3},
The aim of this study was to compare the genetic diversity of the single copy Bv80 gene sequences of Babesia bovis in populations of attenuated and virulent parasites. PCR/ RT-PCR followed by cloning and sequence analyses of 4 attenuated and 4 virulent strains were performed. Multiple fragments in the range of 420 to 744 bp were amplified by PCR or RT-PCR. Cloning of the PCR fragments and sequence analyses revealed the presence of mixed subpopulations in either virulent or attenuated parasites… CONTINUE READING
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