Genetic diversity and phylogeny of rhizobia isolated from agroforestry legume species in southern Ethiopia.

@article{Woldemeskel2005GeneticDA,
  title={Genetic diversity and phylogeny of rhizobia isolated from agroforestry legume species in southern Ethiopia.},
  author={Endalkachew Wolde-meskel and Zewdu Terefework and Asa Frosteg{\aa}rd and Kristina Lindstr{\"o}m},
  journal={International journal of systematic and evolutionary microbiology},
  year={2005},
  volume={55 Pt 4},
  pages={1439-52}
}
The genetic diversity within 195 rhizobial strains isolated from root nodules of 18 agroforestry species (15 woody and three herbaceous legumes) growing in diverse ecoclimatic zones in southern Ethiopia was investigated by using PCR-RFLP of the ribosomal operon [16S rRNA gene, 23S rRNA gene and the internal transcribed spacer (ITS) region between the 16S rRNA and 23S rRNA genes] and 16S rRNA gene partial sequence (800 and 1350 bp) analyses. All of the isolates and the 28 reference strains could… CONTINUE READING

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