Genetic diversity and geographical distribution of indigenous soybean-nodulating bradyrhizobia in the United States.

  title={Genetic diversity and geographical distribution of indigenous soybean-nodulating bradyrhizobia in the United States.},
  author={Sokichi Shiro and Syota Matsuura and Rina Saiki and Gilbert C. Sigua and Akihiro Yamamoto and Yosuke Umehara and Masaki Hayashi and Yuichi Saeki},
  journal={Applied and environmental microbiology},
  volume={79 12},
We investigated the relationship between the genetic diversity of indigenous soybean-nodulating bradyrhizobia and their geographical distribution in the United States using nine soil isolates from eight states. The bradyrhizobia were inoculated on three soybean Rj genotypes (non-Rj, Rj(2)Rj(3), and Rj(4)). We analyzed their genetic diversity and community structure by means of restriction fragment length polymorphisms of PCR amplicons to target the 16S-23S rRNA gene internal transcribed spacer… CONTINUE READING
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