Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.

  title={Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.},
  author={Per Jonasson and Sissela Liljeqvist and P {\AA} Nygren and Stefan St{\aa}hl},
  journal={Biotechnology and applied biochemistry},
  volume={35 Pt 2},
Genetic strategies have been used for more than two decades to improve bacterial bioprocesses and to simplify recovery procedures. Such strategies include the design of efficient expression vectors and the improvement of bacterial production strains in different ways, e.g. by deletion of protease genes or engineering for overexpression of rare-codon tRNAs, foldases or chaperones. Gene multimerization is another such principle that has proved beneficial to improve production yields. Genetic… CONTINUE READING
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