Genetic content of wild-type human cytomegalovirus.

@article{Dolan2004GeneticCO,
  title={Genetic content of wild-type human cytomegalovirus.},
  author={Aidan Dolan and Charles Cunningham and Ralph D Hector and Aycan F. Hassan-Walker and Lydia Lee and Clare Addison and Derrick J. Dargan and Duncan J. McGeoch and Derek Gatherer and Vincent C. Emery and Paul D. Griffiths and Christian Sinzger and Brian Patrick McSharry and Gavin W G Wilkinson and Andrew J. Davison},
  journal={The Journal of general virology},
  year={2004},
  volume={85 Pt 5},
  pages={
          1301-12
        }
}
The genetic content of wild-type human cytomegalovirus was investigated by sequencing the 235 645 bp genome of a low passage strain (Merlin). Substantial regions of the genome (genes RL1-UL11, UL105-UL112 and UL120-UL150) were also sequenced in several other strains, including two that had not been passaged in cell culture. Comparative analyses, which employed the published genome sequence of a high passage strain (AD169), indicated that Merlin accurately reflects the wild-type complement of… 
View on PubMed
doi.org
534 Citations
Highly Influential Citations
44
Background Citations
244
Methods Citations
41
Results Citations
15

Figures and Tables from this paper

534 Citations

Citation Type

Citation Type

Genetic Variability of Human Cytomegalovirus UL132 Gene in Strains from Infected Infants
TLDR
The results showed that a large number of nucleotide non‐synonymous substitutions occurred in the UL132 ORF, particularly in the 5′ half, in comparison to the UL 132 of reference strain, Toledo.
Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture
TLDR
Results suggest strongly that RL13 and UL128L exert at least partially independent suppressive effects on growth in fibroblasts, particularly those involved in cell tropism, immune evasion or growth temperance.
High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169
TLDR
Using high-throughput technology, this work has sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in UL/b′ and the other intact in this region.
Genome sequences of human cytomegalovirus strain TB40/E variants propagated in fibroblasts and epithelial cells
TLDR
The use of PacBio sequencing is reported to elucidate the genetic changes that occurred, both at the consensus level and within subpopulations, upon passaging a TB40/E stock on ARPE-19 epithelial cells and suggests that IE2, and perhaps its interactions with UL84, have important functions unique to HCMV replication in epithelial Cells.
Human cytomegalovirus UL145 gene is highly conserved among clinical strains
TLDR
The structure of the UL145 gene and its putative protein are relatively conserved among clinical strains, irrespective of whether the strains come from patients with different manifestations, from different areas of the world, or were passaged or not in human embryonic lung fibroblast (HELF) cells.
Sequence variability of the α‐chemokine UL146 from clinical strains of human cytomegalovirus
Human cytomegalovirus (HCMV) is a ubiquitous pathogen that infects a variety of cell types in vivo. A region (referred to as UL/b') present in the Toledo strain of HCMV and low passage clinical…
Sequence Variability of Human Cytomegalovirus UL146 and UL147 Genes in Low-Passage Clinical Isolates
TLDR
Sequence variability among HCMV clinical strains may affect the ability of UL146 and UL147 to attract human neutrophils and influence viral dissemination.
Human cytomegalovirus genome.
TLDR
The coding content of clinical isolates is re-evaluated by identifying the set of open reading frames (ORFs) that are conserved in all five sequenced clinical isolate and which of these ORFs are present in the chimpanzee cytomegalovirus (CCMV) genome.
Genetic Stability of Bacterial Artificial Chromosome-Derived Human Cytomegalovirus during Culture In Vitro
TLDR
Conditions under which BAC-derived viruses containing an intact, wild-type genome can be propagated in vitro with minimal risk of mutants being selected are identified, enabling studies of viruses expressing the gene complement of a clinical strain.
Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication.
TLDR
To overcome rapid emergence of mutations in genetically intact HCMV, a system in which RL13 and UL131A were conditionally repressed during virus propagation is developed, which permits studies to be undertaken with a clonal, characterized H CMV strain containing the complete wild-type gene complement.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 58 REFERENCES
A review of genetic differences between limited and extensively passaged human cytomegalovirus strains
TLDR
It is hypothesized that this plasticity of the genome at the right edge of the unique long (UL) component of the UL component results from extensive passage and adaptation to replication in fibroblasts in vitro.
Two novel spliced genes in human cytomegalovirus.
TLDR
Observations indicate that expression of either one of UL128 and UL131A is deleterious to growth of primate cytomegaloviruses in cell culture.
Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains
TLDR
A dramatic level of genome sequence complexity is revealed that may explain the differences that these strains exhibit in virulence and tissue tropism and suggests that wild-type virus carries more than 220 genes, some of which are lost by large-scale deletion and rearrangement of the UL/b' region during laboratory passage.
Human Cytomegalovirus UL144 Open Reading Frame: Sequence Hypervariability in Low-Passage Clinical Isolates
TLDR
The predicted UL144 product homology with tumor necrosis factor receptor family members, along with the unexpectedly high level of sequence variability of the UL144 ORF, suggests that the predicted product may play a role in HCMV infectivity and subsequent host disease.
Functional map of human cytomegalovirus AD169 defined by global mutational analysis
  • Dong Yu, M. Silva, T. Shenk
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2003
TLDR
An infectious bacterial artificial chromosome is used to introduce 413 defined insertion and substitution mutations into the human cytomegalovirus AD169 genome by random and site-directed transposon mutagenesis, producing mutations with a high probability of encoding proteins for which mutants have not been previously documented and in many previously characterized ORFs.
The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome.
TLDR
The sequence (241 087 bp) of chimpanzee cytomegalovirus (CCMV) is determined and compared with published HCMV sequences from the laboratory strains AD169 and Toledo, with the aim of clarifying the gene content of wild-type H CMV.
Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes.
TLDR
Comparisons of sequences near the left end of the genome in several HCMV strains revealed two adjacent open reading frames that potentially encode related proteins: RL6, which is hypervariable, and RL5A, which has not been recognized previously, and thus constitute two new members.
Functional profiling of a human cytomegalovirus genome
  • W. Dunn, C. Chou, +5 authors Fenyong Liu
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 2003
TLDR
HCMV encodes supportive and suppressive growth regulators for optimizing its replication in human fibroblasts, epithelial, and endothelial cells, and may contribute to HCMV's optimal infection of different tissues and successful proliferation among the human population.
Reassessing the organization of the UL42-UL43 region of the human cytomegalovirus strain AD169 genome.
A polymorphism in the UL42-UL43 region of the human cytomegalovirus genome has been characterized by nucleotide sequence analysis, revealing a 929-bp insertion following nt 54,612 relative to the…
The published DNA sequence of human cytomegalovirus strain AD169 lacks 929 base pairs affecting genes UL42 and UL43
TLDR
Compared with the published DNA sequence, most isolates of human cytomegalovirus strain AD169 contain an additional 929 bp after nucleotide 54612, which results in a changed reading frame for the 5'-terminal 50 codons of gene UL42 and expansion of genes UL43 (a US22 family member) from 187 (3'-truncated) to 423 (full-length) codons.
...
1
2
3
4
5
...