Genetic characterization of a single bifunctional enzyme for fumarate reduction and succinate oxidation in Geobacter sulfurreducens and engineering of fumarate reduction in Geobacter metallireducens.

@article{Butler2006GeneticCO,
  title={Genetic characterization of a single bifunctional enzyme for fumarate reduction and succinate oxidation in Geobacter sulfurreducens and engineering of fumarate reduction in Geobacter metallireducens.},
  author={Jessica E. Butler and Richard H. Glaven and Abraham Esteve-N{\'u}{\~n}ez and Cinthia N{\'u}{\~n}ez and Evgenya S. Shelobolina and Daniel R Bond and Derek R. Lovley},
  journal={Journal of bacteriology},
  year={2006},
  volume={188 2},
  pages={450-5}
}
The mechanism of fumarate reduction in Geobacter sulfurreducens was investigated. The genome contained genes encoding a heterotrimeric fumarate reductase, FrdCAB, with homology to the fumarate reductase of Wolinella succinogenes and the succinate dehydrogenase of Bacillus subtilis. Mutation of the putative catalytic subunit of the enzyme resulted in a strain that lacked fumarate reductase activity and was unable to grow with fumarate as the terminal electron acceptor. The mutant strain also… CONTINUE READING