Genetic characterization of a Tn5-disrupted glycosyltransferase gene homolog in Brucella abortus and its effect on lipopolysaccharide composition and virulence.

@article{McQuiston1999GeneticCO,
  title={Genetic characterization of a Tn5-disrupted glycosyltransferase gene homolog in Brucella abortus and its effect on lipopolysaccharide composition and virulence.},
  author={John R. McQuiston and Ramesh Vemulapalli and Thomas J. Inzana and Gerhardt G. Schurig and Nammalwar Sriranganathan and David C. Fritzinger and Ted L. Hadfield and Robin A. Warren and Luther E. Lindler and N J Snellings and David Hoover and Shirley M. Halling and Stephen M. Boyle},
  journal={Infection and immunity},
  year={1999},
  volume={67 8},
  pages={3830-5}
}
We constructed a rough mutant of Brucella abortus 2308 by transposon (Tn5) mutagenesis. Neither whole cells nor extracted lipopolysaccharide (LPS) from this mutant, designated RA1, reacted with a Brucella O-side-chain-specific monoclonal antibody (MAb), Bru-38, indicating the absence of O-side-chain synthesis. Compositional analyses of LPS from strain RA1 showed reduced levels of quinovosamine and mannose relative to the levels in the parental, wild-type strain, 2308. We isolated DNA flanking… CONTINUE READING
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