Genetic breeding of L-tyrosine producer from Brevibacterium lactofermentum.

@article{Ito1990GeneticBO,
  title={Genetic breeding of L-tyrosine producer from Brevibacterium lactofermentum.},
  author={Hiroyuki Ito and Susumu Sakurai and Tatsuo Tanaka and Kazuhiro Sato and Hitoshi Enei},
  journal={Agricultural and biological chemistry},
  year={1990},
  volume={54 3},
  pages={699-705}
}
A wild-type parent of Brevibacterium lactofermentum was converted into an L-Tyr producer by three steps of genetic breeding. First, acquirement of m-fluoro-D, L-phenylalanine resistance (1,000 microgram/ml) brought about MF1317 which produced 3.5 g/l of L-Tyr and a byproduct of 2.8 g/l of L-Phe. Second, increase in the drug resistance (5,000 microgram/ml) gave MF358 that produced 6.4 g/l of L-Tyr and a byproduct of 6.0 g/l of L-Phe. Third, an L-Phe auxotrophic mutant (FT-1) derived from MF358… CONTINUE READING