Genetic basis of hindlimb loss in a naturally occurring vertebrate model.

@article{Don2016GeneticBO,
  title={Genetic basis of hindlimb loss in a naturally occurring vertebrate model.},
  author={Emily K. Don and Tanya A. de Jong-Curtain and Karen Doggett and Thomas Eric Hall and Benjamin Heng and Andrew P. Badrock and Claire G Winnick and Garth A. Nicholson and Gilles J Guillemin and Peter David Currie and Daniel Hesselson and Joan K. Heath and Nicholas J. Cole},
  journal={Biology open},
  year={2016},
  volume={5 3},
  pages={359-66}
}
Here we genetically characterise pelvic finless, a naturally occurring model of hindlimb loss in zebrafish that lacks pelvic fin structures, which are homologous to tetrapod hindlimbs, but displays no other abnormalities. Using a hybrid positional cloning and next generation sequencing approach, we identified mutations in the nuclear localisation signal (NLS) of T-box transcription factor 4 (Tbx4) that impair nuclear localisation of the protein, resulting in altered gene expression patterns… CONTINUE READING