Genetic basis of evolutionary adaptation by Escherichia coli to stressful cycles of freezing, thawing and growth.

@article{Sleight2008GeneticBO,
  title={Genetic basis of evolutionary adaptation by Escherichia coli to stressful cycles of freezing, thawing and growth.},
  author={Sean C. Sleight and Christian Orlic and Dominique Schneider and Richard E. Lenski},
  journal={Genetics},
  year={2008},
  volume={180 1},
  pages={431-43}
}
Microbial evolution experiments offer a powerful approach for coupling changes in complex phenotypes, including fitness and its components, with specific mutations. Here we investigate mutations substituted in 15 lines of Escherichia coli that evolved for 1000 generations under freeze-thaw-growth (FTG) conditions. To investigate the genetic basis of their improvements, we screened many of the lines for mutations involving insertion sequence (IS) elements and identified two genes where multiple… CONTINUE READING