Genetic barcoding facilitates captive and wild management of three cryptic African crocodile species complexes

  title={Genetic barcoding facilitates captive and wild management of three cryptic African crocodile species complexes},
  author={Matthew H. Shirley and Vicki L. Villanova and Kent A Vliet and James D. Austin},
  journal={Animal Conservation},
Management of threatened species with conserved morphology is hindered by misidentification and potential hybridization in captivity. African crocodiles of the genera Osteolaemus, Crocodylus and Mecistops were previously considered monotypic, but have recently been identified as consisting of three, two and two cryptic species, respectively. Mitochondrial cytochrome c oxidase subunit I (COI) new and published sequences from 310 wild‐caught specimens were compared with 71 US zoological specimens… 

Co-occurring cryptic species pose challenges for conservation: a case study of the African dwarf crocodile (Osteolaemus spp.) in Cameroon

Abstract The conservation status of threatened taxa may be obfuscated by the detection of cryptic species complexes, in both vertebrate and invertebrate species. African dwarf crocodiles (Osteolaemus

Mitogenomic investigation reveals a cryptic lineage of Crocodylus in Cuba

The results are consistent with the hypothesis that Antillean C. acutus represents a cryptic lineage with genetic divergence at the species level and provide important information to be integrated into current Crocodylus conservation strategies in Cuba.

Modern cryptic species and crocodylian diversity in the fossil record

  • C. BrochuC. Sumrall
  • Environmental Science, Geography
    Zoological Journal of the Linnean Society
  • 2020
Ranges of variation in modern species require a thorough re-evaluation, and the authors may have to revisit previous perceptions of past crocodyliform diversity, rates of evolution or anagenetic lineages in stratigraphic succession.

Fine scale patterns of genetic partitioning in the rediscovered African crocodile, Crocodylus suchus (Saint-Hilaire 1807)

This work examined genetic variation within the rediscovered African crocodile, Crocodylus suchus, across its distribution in West and Central Africa and identified up to eight genetic clusters that largely correspond to populations isolated in coastal wetland systems and across large distances.

The importance of genetic research in zoo breeding programmes for threatened species: the African dwarf crocodiles (genus Osteolaemus) as a case study

The case study of dwarf crocodiles in zoological institutions reinforces the importance of genetic research in conservation-breeding programmes, highlights the potential for collaboration between European and American zoological Institutions for the ex situ conservation of threatened wildlife, and foreshadows some of the regulatory challenges in managing captive populations internationally.

Delimitation of evolutionary units in Cuvier’s dwarf caiman, Paleosuchus palpebrosus (Cuvier, 1807): insights from conservation of a broadly distributed species

This study tested the hypothesis that Cuvier's dwarf caiman (Paleosuchus palpebrosus)—a species broadly distributed across several biomes and watersheds of South America—is comprised of different ESUs, and suggested that conservation research should not neglect species that are generally considered of Least Concern by IUCN due to the taxon’s broad geographic distribution.

Systematic revision of the living African Slender-snouted Crocodiles (Mecistops Gray, 1844).

A neotype for M. cataphractus is designated and a revised diagnosis for crown Mecistops is provided, and previous descriptions of the two living species are revised and updated, including providing both more complete descriptions and discussion of diagnostic characters.

Conservation Genetics of New World Crocodilians

The chapter gives an introductory overview on the biology of the 11 species of crocodilians inhabiting the Americas. Geographical distribution is broadly discussed in the light of biogeographical and

Phylogenetic analysis of a new morphological dataset elucidates the evolutionary history of Crocodylia and resolves the long-standing gharial problem

A new morphological dataset for Crocodylia is presented based on a critical reappraisal of published crocodylian character data matrices and extensive firsthand observations of a global sample of croc Codylians, which robustly recover Gavialis as more closely related to Tomistoma than to other extant cro codylians for the first time based on morphology alone.



Genetic characterization of captive Cuban crocodiles (Crocodylus rhombifer) and evidence of hybridization with the American crocodile (Crocodylus acutus).

Samples from captive individuals were used to genetically characterize this species in comparison with other New World crocodilians, and microsatellite analyses revealed that most admixed individuals were F(2) hybrids between C. rhombifer-alpha and the American crocodile (C. acutus).

Barcoding bushmeat: molecular identification of Central African and South American harvested vertebrates

The creation and use of a globally available database of DNA sequences from a standardized gene region has been proposed as a tool for species identification, assessing genetic diversity and

Rigorous approaches to species delimitation have significant implications for African crocodilian systematics and conservation

The hypothesis that the slender-snouted crocodile (Mecistops cataphractus) is composed of multiple species corresponding to the Congolian and Guinean biogeographic zones is tested and underscores the necessity of comprehensive phylogeographic analyses within currently recognized taxa to detect cryptic species within the Crocodylia.

Comparing and combining distance‐based and character‐based approaches for barcoding turtles

This study constitutes the first comprehensive barcoding effort for this seriously threatened order of turtles andplementing distance‐based barc coding with character‐based methods for identifying diagnostic sets of nucleotides provided better resolution in several cases where distance-based methods failed to distinguish species.

Hybridization between Crocodylus acutus and Crocodylus moreletii in the Yucatan Peninsula: II. Evidence from microsatellites.

Tests for hybridization among wild populations of American crocodile and Morelet's crocodile in the Yucatan Peninsula did not find a significant decrease in hybridity across three life stages, thus far indicating a low level of selection against hybrids.

An ancient icon reveals new mysteries: mummy DNA resurrects a cryptic species within the Nile crocodile.

A cryptic evolutionary lineage within the Nile crocodile is revealed that elucidates the biogeographic history of the genus and clarifies long-standing arguments over the species' taxonomic identity and conservation status.

Molecular identification of crocodile species using novel primers for forensic analysis

Two novel sets of primers are developed to amplify two partial cytochrome b gene sequences of six crocodile species to give a complete cyt b gene sequence, which can be used as an effective tool for forensic authentication of crocodiles species.

Assessing DNA Barcoding as a Tool for Species Identification and Data Quality Control

This study reveals that erroneous sequences are not rare in GenBank and that the DNA barcoding can serve to confirm sequencing accuracy and discover problems such as misidentified species, inaccurate taxonomies, contamination, and potential errors in sequencing.