Genetic assays to define and characterize protein-protein interactions involved in gene regulation.

@article{Nickels2009GeneticAT,
  title={Genetic assays to define and characterize protein-protein interactions involved in gene regulation.},
  author={Bryce E. Nickels},
  journal={Methods},
  year={2009},
  volume={47 1},
  pages={53-62}
}
Transcription can be regulated during initiation, elongation, and termination by an enormous variety of regulatory factors. A critical step in obtaining a mechanistic understanding of regulatory factor function is the determination of whether the regulatory factor exerts its effect through direct contact with the transcription machinery. Here I describe the application of a transcription activation-based bacterial two-hybrid assay that is useful for the identification and genetic dissection of… CONTINUE READING

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Here I describe the application of a transcription activation - based bacterial two - hybrid assay that is useful for the identification and genetic dissection of protein - protein interactions involved in gene regulation .
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