Genetic and transcriptional analysis of absA, an antibiotic gene cluster‐linked two‐component system that regulates multiple antibiotics in Streptomyces coelicolor

@article{Anderson2001GeneticAT,
  title={Genetic and transcriptional analysis of absA, an antibiotic gene cluster‐linked two‐component system that regulates multiple antibiotics in Streptomyces coelicolor},
  author={Todd B. Anderson and P. Dilkes Brian and Wendy C. Champness},
  journal={Molecular Microbiology},
  year={2001},
  volume={39}
}
In Streptomyces coelicolor, the AbsA1–AbsA2 two‐component system regulates the expression of multiple antibiotic gene clusters. Here, we show that the response regulator encoded by the absA2 gene is a negative regulator of these antibiotic gene clusters. A genetic analysis shows that the phosphorylated form of the AbsA2 response regulator (phospho‐AbsA2), generated by the cognate AbsA1 sensor histidine kinase, is required for normal growth phase regulation of antibiotic synthesis. In the… Expand
Regulation of the Streptomyces coelicolor Calcium-Dependent Antibiotic by absA, Encoding a Cluster-Linked Two-Component System
TLDR
This paper has identified numerous transcriptional start sites within the CDA cluster and shown that the original antibiotic-negative mutants used to identify absA exhibit a stronger negative regulation of promoters upstream of the proposed CDA biosynthetic genes than of promoters in the clusters responsible for production of actinorhodin and undecylprodigiosin. Expand
Identification of different promoters in the absA1–absA2 two-component system, a negative regulator of antibiotic production in Streptomyces coelicolor
TLDR
It is shown by different technical approaches that the absA1–absA2 operon is transcribed from at least two promoters, the first producing a read-through transcript that includes both abs a1 and absA2 genes and the second including only the abs a2 gene. Expand
Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
TLDR
The data suggest that AbsA2 approximately P inhibits antibiotic production by directly interfering with the expression of pathway-specific regulators of antibiotic biosynthetic gene clusters. Expand
Genome-wide analysis of the role of the antibiotic biosynthesis regulator AbsA2 in Streptomyces coelicolor A3(2)
TLDR
The analysis of a ΔabsA2 deletion strain, which exhibits the classic precocious antibiotic hyper-production phenotype, and its complementation by an N-terminal triple-FLAG-tagged version of AbsA2 sheds light on the complex mechanism of regulation of antibiotic biosynthesis in Streptomyces coelicolor. Expand
Genome-wide analysis of the role of the antibiotic biosynthesis regulator AbsA2 in Streptomyces coelicolor A3(2)
TLDR
The analysis of a ΔabsA2 deletion strain, which exhibits the classic precocious antibiotic hyper-production phenotype, and its complementation by an N-terminal triple-FLAG-tagged version of AbsA2 sheds light on the complex mechanism of regulation of antibiotic biosynthesis in Streptomyces coelicolor. Expand
Biochemical Activities of the absA Two-Component System of Streptomyces coelicolor
TLDR
The results support a model in which the phosphorylation state of AbsA2 is determined by the balance of the kinase and phosphatase activities of absA1 and where AbsA 2 approximately P represses antibiotic biosynthetic genes either directly or indirectly. Expand
Transcriptional activation of the pathway‐specific regulator of the actinorhodin biosynthetic genes in Streptomyces coelicolor
TLDR
Findings provide further evidence that the path leading to the expression of pathway‐specific activators of antibiotic biosynthesis genes in disparate Streptomyces may share evolutionarily conserved components in at least some cases, and suggests that the regulation of streptomycin production, which serves an important paradigm, may be more complex than represented by current models. Expand
Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays.
TLDR
A knowledge-based algorithm that correlates temporal changes in expression with chromosomal position identified groups of contiguous genes expressed at discrete stages of morphological development, inferred the boundaries of known antibiotic synthesis gene loci, and revealed novel physical clusters of coordinately regulated genes. Expand
Cross‐regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor
TLDR
A network of functional interactions among regulators that govern production of antibiotics and other secondary metabolites in S. coelicolor are revealed, which suggest that revision of the currently prevalent view of higher‐level versus pathway‐specific regulation of secondary metabolism in Streptomyces species is warranted. Expand
Identification of a Gene Negatively Affecting Antibiotic Production and Morphological Differentiation in Streptomyces coelicolor A3(2)
TLDR
The subcloning of SC7A1 insert DNA resulted in the identification of the open reading frame SCO5582 as nsdA, a gene negatively affecting Streptomyces differentiation that caused the overproduction of spores and of three of four known S. coelicolor antibiotics of quite different chemical types. Expand
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It is proposed that the absA locus encodes a signal transduction mechanism that negatively regulates synthesis of the multiple antibiotics produced by S. coelicolor. Expand
Transcriptional Regulation of Streptomyces coelicolorPathway-Specific Antibiotic Regulators by the absA andabsB Loci
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The results establish that the abs genes affect production of antibiotics through regulation of expression of the antibiotic-specific regulatory genes in S. coelicolor through regulating transcript abundance from the biosynthetic and regulatory genes specific for each antibiotic. Expand
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TLDR
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