Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting.

@article{Rong1994GeneticAP,
  title={Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting.},
  author={J C Rong and John D. Helmann},
  journal={Journal of bacteriology},
  year={1994},
  volume={176 17},
  pages={5218-24}
}
The Bacillus subtilis sigA gene encodes the primary sigma factor of RNA polymerase and is essential for cell growth. We have mutated conserved region 2.3 of the sigma A protein to substitute each of seven aromatic amino acids with alanine. Several of these aromatic amino acids are proposed to form a melting motif which facilitates the strand separation step of initiation. Holoenzymes containing mutant sigma factors recognize promoters, but some are defective for DNA melting in vitro. We have… CONTINUE READING

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Promoter melting triggered by bacterial RNA polymerase occurs in three steps.

Proceedings of the National Academy of Sciences of the United States of America • 2010

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