Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli.

@article{Humbert1980GeneticAB,
  title={Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli.},
  author={R. Humbert and Robert D. Simoni},
  journal={Journal of bacteriology},
  year={1980},
  volume={142 1},
  pages={
          212-20
        }
}
Genetic experiments have indicated that asparagine auxotrophs of Escherichias coli K-12 can be made asparagine prototrophs at either of two sites on the chromosome and that wild-type strains require both sites to be mutated to produce asparagine auxotrophy. The former asn locus is now called asnA, and the new gene is designated asnB. The asnB gene is located near gal.AsnA+ asnB and asnA asnB+ strains were constructed, and the asparagine synthetic reaction was characterized in extracts. These… CONTINUE READING

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