Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast.

@article{Uno1983GeneticAB,
  title={Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast.},
  author={Isao Uno and Kunihiro Matsumoto and Kazuhiko Adachi and Takahiko Ishikawa},
  journal={The Journal of biological chemistry},
  year={1983},
  volume={258 18},
  pages={10867-72}
}
In Saccharomyces cerevisiae, trehalase activity in crude extracts obtained from wild type cells was activated about 3-fold by preincubation with cAMP and ATP. The inactive trehalase fractionated by DEAE-Sephacel chromatography was activated by the addition of the cAMP-dependent protein kinase fraction from wild type cells in the presence of cAMP and ATP. Using the crude extract obtained from bcy1 mutant cells which were deficient in the regulatory subunit of cAMP-dependent protein kinase, the… CONTINUE READING

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