Genetic and biochemical characterization of the aerobactin synthesis operon on pColV

  title={Genetic and biochemical characterization of the aerobactin synthesis operon on pColV},
  author={Roy Gross and Fred Engelbrecht and Volkmar Braun},
  journal={Molecular and General Genetics MGG},
Certain ColV plasmids of Escherichia coli contain genes that specify functions for the acquisition of iron-(III) via aerobactin. The locus for aerobactin synthesis of pColV-K311 was cloned into pBR322. Mutagenesis with the transposon Tn1000, and the generation of deletions with restriction enzymes resulted in multicopy plasmids which complemented pColV mutants impaired in various steps of aerobactin synthesis. The insertion and deletion mutants were mapped and assigned to three loci termed aerA… CONTINUE READING


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