Genetic and biochemical analysis of the fission yeast ribonucleoprotein particle containing a homolog of Srp54p

@article{Selinger1994GeneticAB,
  title={Genetic and biochemical analysis of the fission yeast ribonucleoprotein particle containing a homolog of Srp54p},
  author={David Selinger and Patrick J. Brennwald and Steven Althoff and Claudia Reich and Byron C. Hann and Peter Walter and Jo Ann Wise},
  journal={Nucleic acids research},
  year={1994},
  volume={22 13},
  pages={
          2557-67
        }
}
Mammalian signal recognition particle (SRP), a complex of six polypeptides and one 7SL RNA molecule, is required for targeting nascent presecretory proteins to the endoplasmic reticulum (ER). Earlier work identified a Schizosaccharomyces pombe homolog of human SRP RNA and showed that it is a component of a particle similar in size and biochemical properties to mammalian SRP. The recent cloning of the gene encoding a fission yeast protein homologous to Srp54p has made possible further… 

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References

SHOWING 1-10 OF 53 REFERENCES
Biochemistry
  • F. Young
  • Education
    The Indian Medical Gazette
  • 1955
The Department of Biochemistry is internationally recognized for its research and education and offers a world-class interdisciplinary research environment in a beautiful mountain setting. As part of
Molecular Cloning A Laboratory Manual Second Edition Sambrook
Cell
Mo. Cell. Biol
  • Mo. Cell. Biol
  • 1990
Submitted for publication Molecular Cloning: a Laboratory Manual
  • Submitted for publication Molecular Cloning: a Laboratory Manual
  • 1989
Proc. Natl. Acad. Sci. USA
  • Proc. Natl. Acad. Sci. USA
  • 1989
J. Cell Biol
  • J. Cell Biol
  • 1989
Nucleic Acids Res
  • Nucleic Acids Res
  • 1992
Proc. Natl. Acad. Sci. USA
  • Proc. Natl. Acad. Sci. USA
  • 1980
Nucleic Acids Res
  • Nucleic Acids Res
  • 1985
...
...