Genetic analysis of varicella-zoster virus ORF0 to ORF4 by use of a novel luciferase bacterial artificial chromosome system.

@article{Zhang2007GeneticAO,
  title={Genetic analysis of varicella-zoster virus ORF0 to ORF4 by use of a novel luciferase bacterial artificial chromosome system.},
  author={Zhen Zhang and Jenny Rowe and Weijia Wang and Marvin Sommer and Ann H Arvin and Jennifer F. Moffat and Hua Zhu},
  journal={Journal of virology},
  year={2007},
  volume={81 17},
  pages={9024-33}
}
To efficiently generate varicella-zoster virus (VZV) mutants, we inserted a bacterial artificial chromosome (BAC) vector in the pOka genome. We showed that the recombinant VZV (VZV(BAC)) strain was produced efficiently from the BAC DNA and behaved indistinguishably from wild-type virus. Moreover, VZV's cell-associated nature makes characterizing VZV mutant growth kinetics difficult, especially when attempts are made to monitor viral replication in vivo. To overcome this problem, we then created… CONTINUE READING