Genetic analysis of type 5 capsular polysaccharide expression by Staphylococcus aureus.

@article{Lee1994GeneticAO,
  title={Genetic analysis of type 5 capsular polysaccharide expression by Staphylococcus aureus.},
  author={J. Ching Lee and Shunbin Xu and Anne Albus and P J Livolsi},
  journal={Journal of bacteriology},
  year={1994},
  volume={176 16},
  pages={4883-9}
}
Capsules are produced by over 90% of Staphylococcus aureus strains, and approximately 25% of clinical isolates express type 5 capsular polysaccharide (CP5). We mutagenized the type 5 strain Reynolds with Tn918 to target genes involved in CP5 expression. From a capsule-deficient mutant, we cloned into a cosmid vector an approximately 26-kb EcoRI fragment containing the transposon insertion. In the absence of tetracycline selection, Tn918 was spontaneously excised, thereby resulting in a plasmid… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

Similar Papers

Loading similar papers…