Genetic analysis of the pestivirus nonstructural coding region: defects in the NS5A unit can be complemented in trans.

@article{Grassmann2001GeneticAO,
  title={Genetic analysis of the pestivirus nonstructural coding region: defects in the NS5A unit can be complemented in trans.},
  author={Claus Wilhelm Grassmann and Olaf Isken and Norbert Tautz and Sven Erik Behrens},
  journal={Journal of virology},
  year={2001},
  volume={75 17},
  pages={7791-802}
}
The functional analysis of molecular determinants which control the replication of pestiviruses was considerably facilitated by the finding that subgenomic forms of the positive-strand RNA genome of BVDV (bovine viral diarrhea virus) are capable of autonomous replication in transfected host cells. The prototype replicon, BVDV DI9c, consists of the genomic 5' and 3' untranslated regions and a truncated open reading frame (ORF) encoding mainly the nonstructural proteins NS3, NS4A, NS4B, NS5A, and… CONTINUE READING