Genetic analysis of the gene cluster encoding nonfimbrial adhesin I from an Escherichia coli uropathogen.

@article{Ahrens1993GeneticAO,
  title={Genetic analysis of the gene cluster encoding nonfimbrial adhesin I from an Escherichia coli uropathogen.},
  author={Rebecca Ahrens and Michael Ott and Alice Ritter and H Hoschuetzky and Thomas Buehler and Friedrich Lottspeich and Graham J. Boulnois and Klaus B. Jann and Jorg Hacker},
  journal={Infection and immunity},
  year={1993},
  volume={61 6},
  pages={2505-12}
}
The chromosomally encoded nonfimbrial adhesion I (NFA-I) from Escherichia coli urinary tract isolate 827 (O83:K1:H4) mediates agglutination of human erythrocytes. Subclones were constructed from an NFA-I-expressing recombinant E. coli K-12 clone, derived from a genomic library of E. coli 827. Minicell analysis and nucleotide sequencing revealed that proteins of 30.5, 9, 80, 15, and 19 kDa encoded on a stretch of approximately 6 kb are involved in the expression of NFA-I. NFA-I exhibits a… CONTINUE READING
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