Genetic analysis of sea-ice bacterial communities of the Western Baltic Sea using an improved double gradient method

@article{Petri2001GeneticAO,
  title={Genetic analysis of sea-ice bacterial communities of the Western Baltic Sea using an improved double gradient method},
  author={Ralf Petri and Johannes F Imhoff},
  journal={Polar Biology},
  year={2001},
  volume={24},
  pages={252-257}
}
The bacterial diversity of sea ice from Kiel Bight obtained during the rare event of solid ice cover in spring 1996 was analysed by molecular genetic approaches using an improved double gradient denaturing gradient gel electrophoretic method (DG-DGGE) to separate 16S rDNA fragments of approximately 500 bp. The excellent separation of individual bands within these gradient gels allowed us to obtain sequence information and to allocate the phylogenetic position of representative bacteria from the… CONTINUE READING