Genetic analysis of natural recombinant Brazilian Toxoplasma gondii strains by multilocus PCR-RFLP.

@article{Ferreira2006GeneticAO,
  title={Genetic analysis of natural recombinant Brazilian Toxoplasma gondii strains by multilocus PCR-RFLP.},
  author={Adriana de Melo Ferreira and Ricardo Wagner de Almeida V{\'i}tor and Ricardo Tostes Gazzinelli and Maria Norma de Melo},
  journal={Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases},
  year={2006},
  volume={6 1},
  pages={22-31}
}
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) at eight independent loci was used to determine the types (I, II or III) lineage of 20 Toxoplasma gondii strains isolated from humans and animals in Brazil. RH (type I, highly virulent), ME49 (type II, avirulent) and VEG (type III, avirulent) were used as reference strains. Differently from expected frequencies, all Brazilian strains showed to have recombinant genotypes, with typical alleles of types I, II or III at… CONTINUE READING

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