Genetic analysis of kernel hardness in bread wheat using PCR-based markers

  title={Genetic analysis of kernel hardness in bread wheat using PCR-based markers},
  author={A. A. Galande and Ratan Tiwari and J. S. S. Ammiraju and Dipak K. Santra and Meena D. Lagu and V. S. Prakasa Rao and Vidya S. Gupta and B. K. Misra and Shanta Nagarajan and Prabhakar K. Ranjekar},
  journal={Theoretical and Applied Genetics},
In wheat, kernel hardness is a complex genetic trait involving various directly and indirectly contributing components such as kernel hardness per se, protein content, hectolitre weight and 1,000-kernel weight. In an attempt to identify DNA markers associated with this trait, 100 recombinant inbred lines (RILs) derived from a cross between a hard grain land-race, NP4, and a soft grain variety, HB 208, were screened with 100 ISSR and 360 RAPD primers. Eighteen markers were assigned to seven… CONTINUE READING
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