Genetic analysis of a type IV pili-like locus in the archaeon Methanococcus maripaludis

@article{Nair2014GeneticAO,
  title={Genetic analysis of a type IV pili-like locus in the archaeon Methanococcus maripaludis},
  author={Divya B. Nair and Kaoru Uchida and Shin-ichi Aizawa and Ken F Jarrell},
  journal={Archives of Microbiology},
  year={2014},
  volume={196},
  pages={179-191}
}
Methanococcus maripaludis is a stringently anaerobic archaeon with two studied surface structures, archaella and type IV pili. Previously, it was shown that three pilin genes (mmp0233 [epdA], mmp0236 [epdB] and mmp0237 [epdC]) located within an 11 gene cluster in the genome were necessary for normal piliation. This study focused on analysis of the remaining genes to determine their potential involvement in piliation. Reverse transcriptase PCR experiments demonstrated the 11 genes formed a… CONTINUE READING

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We identified genes for an assembly ATPase and two versions of the conserved pilin platform forming protein necessary for pili assembly at a separate genetic locus .
Methanococcus maripaludis is a stringently anaerobic archaeon with two studied surface structures , archaella and type IV pili .
Methanococcus maripaludis is a stringently anaerobic archaeon with two studied surface structures , archaella and type IV pili .
Reverse transcriptase PCR experiments demonstrated the 11 genes formed a single transcriptional unit .
Reverse transcriptase PCR experiments demonstrated the 11 genes formed a single transcriptional unit .
We identified genes for an assembly ATPase and two versions of the conserved pilin platform forming protein necessary for pili assembly at a separate genetic locus .
Reverse transcriptase PCR experiments demonstrated the 11 genes formed a single transcriptional unit .
Reverse transcriptase PCR experiments demonstrated the 11 genes formed a single transcriptional unit .
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