Genetic analysis of a gene cluster for cyclohexanol oxidation in Acinetobacter sp. Strain SE19 by in vitro transposition.

@article{Cheng2000GeneticAO,
  title={Genetic analysis of a gene cluster for cyclohexanol oxidation in Acinetobacter sp. Strain SE19 by in vitro transposition.},
  author={Qiong Cheng and Sheena M Thomas and K. Kostichka and James R. Valentine and Venugopalan Nagarajan},
  journal={Journal of bacteriology},
  year={2000},
  volume={182 17},
  pages={4744-51}
}
Biological oxidation of cyclic alcohols normally results in formation of the corresponding dicarboxylic acids, which are further metabolized and enter the central carbon metabolism in the cell. We isolated an Acinetobacter sp. from an industrial wastewater bioreactor that utilized cyclohexanol as a sole carbon source. A cosmid library was constructed from Acinetobacter sp. strain SE19, and oxidation of cyclohexanol to adipic acid was demonstrated in recombinant Escherichia coli carrying a SE19… CONTINUE READING