Genetic analysis of DNA excreted in urine: a new approach for detecting specific genomic DNA sequences from cells dying in an organism.

@article{Botezatu2000GeneticAO,
  title={Genetic analysis of DNA excreted in urine: a new approach for detecting specific genomic DNA sequences from cells dying in an organism.},
  author={Irina V. Botezatu and Ol'ga Serdyuk and Galina I. Potapova and Valery P. Shelepov and R P Alechina and Yu. K. Molyaka and V Anan{\'e}v and Ingrid Bazin and Alexandre Garin and M. N. Narimanov and V. I. Knysh and Hovsep S Melkonyan and Samuil Umansky and Anatoly Lichtenstein},
  journal={Clinical chemistry},
  year={2000},
  volume={46 8 Pt 1},
  pages={1078-84}
}
BACKGROUND Cell-free DNA from dying cells recently has been discovered in human blood plasma. In experiments performed on animals and humans, we examined whether this cell-free DNA can cross the kidney barrier and be used as a diagnostic tool. METHODS Mice received subcutaneous injections of either human Raji cells or purified (32)P-labeled DNA. DNA was isolated from urine and analyzed by measurement of radioactivity, agarose gel electrophoresis, and PCR. In humans, the permeability of the… CONTINUE READING