Genetic analysis of Arabidopsis mutants.

  title={Genetic analysis of Arabidopsis mutants.},
  author={Detlef Weigel and Jane Glazebrook},
  journal={CSH protocols},
INTRODUCTIONThe inheritance of mutant phenotypes in Arabidopsis thaliana is most often analyzed in the progeny resulting from crosses between different parents. This article describes genetic strategies for the analysis of mutants. Newly identified mutations can be examined initially using segregation analysis, followed by backcrossing and cosegregation for removal of extraneous mutations and assessment of pleiotropy. Confirmation that a phenotype of interest results from a given mutation can… CONTINUE READING

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