Corpus ID: 212552955

Genetic Expression of High Nitric Oxide Adapted Human Lung Adenocarcinoma Cell Line towards Angiogenesis

@inproceedings{Batool2017GeneticEO,
  title={Genetic Expression of High Nitric Oxide Adapted Human Lung Adenocarcinoma Cell Line towards Angiogenesis},
  author={Khatja Batool and Juel Chowdhury and F Siddiqui and Abhinaya Baskaran and Umar Ahmad and Mahsa Vahdatian and James A. Radosevich},
  year={2017}
}
A549-HNO cells which adapt to high nitric oxide (HNO) proliferate at a higher rate and are more resistant to radiation when compared to A549 parent cells. This is due to an altered expression in several genes where key pathways are affected such as the process of apoptosis, cell proliferation and DNA transcription. A549 lung cancer cells that adapt to high concentrations of nitric oxide (NO) cause new mutations to occur by altering the cell’s genome towards the angiogenesis pathway. The purpose… Expand
2 Citations
S‐nitrosylation of cytoskeletal proteins
TLDR
It is found that S‐nitrosylation of cytoskeletal targets has complementary but distinct effects to cyclic‐GMP in motile and contractile cells—promoting cell migration, and biasing muscle contraction toward relaxation. Expand
Genetic Expression of High Nitric Oxide Adapted Human Lung Adenocarcinoma Cell Line towards Angiogenesis
A549-HNO cells which adapt to high nitric oxide (HNO) proliferate at a higher rate and are more resistant to radiation when compared to A549 parent cells. This is due to an altered expression inExpand

References

SHOWING 1-10 OF 63 REFERENCES
Part I—mechanism of adaptation: high nitric oxide adapted A549 cells show enhanced DNA damage response and activation of antiapoptotic pathways
TLDR
The results here suggest that the HNO adapted A549 cells have increased activation of DNA damage response pathway proteins which can lead to better DNA repair function. Expand
Part I. Molecular and cellular characterization of high nitric oxide-adapted human breast adenocarcinoma cell lines
TLDR
Characterizing the HNO cells and their biological attributes against those of the parent cells provides evidence that cancer cells subjected to HNO concentrations become resistant to free radicals such as NO via up-regulated cellular defense mechanisms, including p53 and GST-pi. Expand
Long-term adaptation of breast tumor cell lines to high concentrations of nitric oxide
TLDR
These cell lines serve as a novel tool to study the role of NO in breast cancer progression and potentially can be used to predict the therapeutic response leading to more efficient therapeutic regimens. Expand
Expression of cross-tolerance to a wide range of conditions in a human lung cancer cell line after adaptation to nitric oxide
TLDR
The results show that the A549-HNO cells exhibit greater viability than the A550-parent cells when exposed to each of the various conditions, suggesting that NO● is one potential driving force that can make tumor cells exhibit cross-tolerance. Expand
Long-term adaptation of the human lung tumor cell line A549 to increasing concentrations of hydrogen peroxide
TLDR
The findings suggest that any free radical can induce resistance to other free radicals; this is especially important given that radiation therapy and many chemotherapeutic agents exert their effect via free radicals. Expand
Long-Term Adaptation of Lung Tumor Cell Lines with Increasing Concentrations of Nitric Oxide Donor
TLDR
An in vitro model system to study how elevated amounts of nitric oxide affect tumor development and propagation in lung tumor cells is developed and adapted cells found to grow faster than the parent cells under both normal growth conditions and stressful growth conditions. Expand
Part I. Development of a model system for studying nitric oxide in tumors: high nitric oxide-adapted head and neck squamous cell carcinoma cell lines
TLDR
The development of an in vitro model system which can be used to probe the role of NO in the carcinogenesis of HNSCC and indicates that the HNO cell lines are unique and possess biologically different properties than the parent cell lines from which they originated. Expand
Betulinic acid inhibits the expression of hypoxia-inducible factor 1α and vascular endothelial growth factor in human endometrial adenocarcinoma cells
TLDR
The data suggest that BA may have anti-angiogenic potential by inhibition of prolidase, HIF-1α and VEGF expressions, and inhibition of collagen biosynthesis. Expand
Part II—mechanism of adaptation: A549 cells adapt to high concentration of nitric oxide through bypass of cell cycle checkpoints
TLDR
Molecular mechanisms involved in cell cycle regulation in A549-HNO cells, which suggest an initial bypass of cell cycle checkpoints as p21CIP1 can inhibit the activity of all cyclin/Cdk complexes, are studied. Expand
A549 cells adapted to high nitric oxide show reduced surface CEACAM expression and altered adhesion and migration properties
TLDR
The adhesion and migration assays showed reduced clumping in HNO-adapted A549 (A549-HNO) cells and faster migration rates, respectively, which document the altered adhesionand migration properties of cells adapted to HNO. Expand
...
1
2
3
4
5
...