Genetic Analysis of a Chromosomal Region Containing Genes Required for Assimilation of Allantoin Nitrogen and Linked Glyoxylate Metabolism in Escherichia coli

@article{Cusa1999GeneticAO,
  title={Genetic Analysis of a Chromosomal Region Containing Genes Required for Assimilation of Allantoin Nitrogen and Linked Glyoxylate Metabolism in Escherichia coli},
  author={Eva Cusa and N. Obradors and L. Baldom{\`a} and J. Badía and J. Aguilar},
  journal={Journal of Bacteriology},
  year={1999},
  volume={181},
  pages={7479 - 7484}
}
ABSTRACT Growth experiments with Escherichia coli have shown that this organism is able to use allantoin as a sole nitrogen source but not as a sole carbon source. Nitrogen assimilation from this compound was possible only under anaerobic conditions, in which all the enzyme activities involved in allantoin metabolism were detected. Of the nine genes encoding proteins required for allantoin degradation, only the one encoding glyoxylate carboligase (gcl), the first enzyme of the pathway leading… Expand
Transcriptional Regulation of the Gene Cluster Encoding Allantoinase and Guanine Deaminase in Klebsiella pneumoniae
TLDR
The cluster encompassing genes KPN_01787 to KPN-01791 is characterized, which is involved in the conversion of allantoin into allantoate and in the deamination of guanine to xanthine and may have a dual role, acting as a repressor in the absence ofallantoin and as an activator in its presence. Expand
Characterization of the gene cluster involved in allantoate catabolism and its transcriptional regulation by the RpiR-type repressor HpxU in Klebsiella pneumoniae.
TLDR
Allantoate released the HpxU repressor from its target operators whereas other purine intermediate metabolites, such as allantoin and oxamate, failed to induce complex dissociation. Expand
The hpx Genetic System for Hypoxanthine Assimilation as a Nitrogen Source in Klebsiella pneumoniae: Gene Organization and Transcriptional Regulation
TLDR
The proteins involved in the oxidation of hypoxanthine to allantoin or uric acid did not display any similarity to other reported enzymes known to catalyze these reactions but instead are similar to oxygenases acting on aromatic compounds. Expand
Biochemical characterization of the 2-ketoacid reductases encoded by ycdW and yiaE genes in Escherichia coli.
TLDR
The similarity with YiaE led us to test whether this protein was responsible for the remaining glyoxylate reductase activity, and purification of YcdW and YIAE proteins permitted their kinetic characterization and comparison. Expand
Functional Analysis of 14 Genes That Constitute the Purine Catabolic Pathway in Bacillus subtilis and Evidence for a Novel Regulon Controlled by the PucR Transcription Activator
TLDR
Allantoic acid, allantoin, and uric acid were all found to function as effector molecules for PucR-dependent regulation of puc gene expression, suggesting that the 14 genes and the gde gene constitute a regulon controlled by the pucR gene product. Expand
Purine Utilization by Klebsiella oxytoca M5al: Genes for Ring-Oxidizing and -Opening Enzymes
TLDR
A 23-gene cluster is identified that encodes the enzymes for utilizing purines as the sole nitrogen source in the enterobacterium Klebsiella oxytoca, and functions for the products of these genes are revealed. Expand
AllR Controls the Expression of Streptomyces coelicolor Allantoin Pathway Genes
TLDR
Genetics and proteomics analysis revealed that among all genes from the allantoin pathway that are upregulated in the allR mutant, the hyi gene encoding a hydroxypyruvate isomerase (Hyi) is responsible of the impairment of antibiotic production. Expand
The Burkholderia cepacia fur gene: co-localization with omlA and absence of regulation by iron.
TLDR
Interestingly, the presence of the divergently transcribed omlA/smpA gene upstream of fur in some members of the gamma subclass of the Proteobacteria is retained in several genera within the beta taxon, including Burkholderia. Expand
The soluble transhydrogenase UdhA affecting the glutamate-dependent acid resistance system of Escherichia coli under acetate stress
TLDR
It was demonstrated that during growth on acetate, the expression of genes involved in the respiratory chain and Gad acid resistance system was inhibited in the udhA-knockout strain and UdhA is an important source of NADH of E.coli in acetate environment. Expand
Laboratory evolution reveals the metabolic and regulatory basis of ethylene glycol metabolism by Pseudomonas putida KT2440.
TLDR
The genomic and metabolic basis of ethylene glycol metabolism in Pseudomonas putida KT2440 is revealed, which provides insights into the environmental fate of this pollutant, but also enables its utilization as a carbon source for microbial biotechnology. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 33 REFERENCES
Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family.
TLDR
The cloning, genomic location, and DNA sequence of the gene (called gcl) encoding E. coli Gcl is reported and a gene (orf258) immediately downstream of the gcl gene encoded a protein (Orf258) of 258 residues, which is consistent with a grouping of Gcl with the AHAS and pyruvate oxidase enzymes. Expand
Regulation of Glyoxylate Metabolism in Escherichia coli K-12
TLDR
Evidence indicates that malate synthase G plays an anaplerotic role during growth with glycolate or acetate as the carbon source, and that wild-type organisms convert glyoxylate to acetyl-coenzyme A and oxidize it via the tricarboxylic acid cycle. Expand
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization
TLDR
In-frame, unmarked deletions are among the most reliable types of mutations available for wild-type E. coli and may be used in competition with one another to reveal phenotypes not apparent when cultured singly. Expand
Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme
TLDR
The functioning of this enzyme results in the regeneration of nicotinamide adenine dinucleotide, which is indistinguishable from the propanediol oxidoreductase induced by anaerobic growth on fucose. Expand
The complete genome sequence of Escherichia coli K-12.
TLDR
The 4,639,221-base pair sequence of Escherichia coli K-12 is presented and reveals ubiquitous as well as narrowly distributed gene families; many families of similar genes within E. coli are also evident. Expand
The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library
TLDR
The strategy was to measure the sizes of partial restriction enzyme digests by hybridization with a vector probe in a manner analogous to nucleotide sequencing, and found that this strategy is applicable to analyses of the genomes of other organisms. Expand
Streptococcus allantoicus and the Fermentation of Allantoin
Only a few kinds of bacteria are known to satisfy their energy requirements by decomposing single nitrogenous compounds under anaerobic conditions. These are Clostridium tetanomorphum and C.Expand
A method for constructing single-copy lac fusions in Salmonella typhimurium and its application to the hemA-prfA operon
  • T. Elliott
  • Medicine, Biology
  • Journal of bacteriology
  • 1992
TLDR
A transposable version of the put operon was constructed and used to direct lac fusions to novel locations, including the F plasmid and the ara locus, and the contribution of the hemA promoter region to expression of the prfA gene and other genes downstream of hemA in S. typhimurium. Expand
Improved single and multicopy lac-based cloning vectors for protein and operon fusions.
TLDR
Several new vectors for the construction of operon and protein fusions to the Escherichia coli lacZ gene are described, improved in that they have very low levels of background lac gene expression, which makes possible the easy detection and accurate quantitation of very weak transcriptional and translational signals. Expand
A short course in bacterial genetics
TLDR
This second edition of Molecular Cloning reflects more comprehensive coverage of topics previously included in the first edition as well as the addition of new chapters, such as those on oligonucleotide probes and mutagenesis, in vitro amplification by the polymerase chain reaction, expression of cloned genes in Escherichia coli and mammalian cells, and analysis of proteins ex­ pressed from cloning genes. Expand
...
1
2
3
4
...