Genetic Analysis of Israel Acute Paralysis Virus: Distinct Clusters Are Circulating in the United States

  title={Genetic Analysis of Israel Acute Paralysis Virus: Distinct Clusters Are Circulating in the United States},
  author={Gustavo F. Palacios and Jia Hui and Phuong-Lan Quan and Abby L. Kalkstein and K S Honkavuori and Ana Valeria Bussetti and Sean Conlan and J. D. Evans and Y. P. Chen and Dennis vanEngelsdorp and Haim Efrat and Jeffery S. Pettis and Diana Cox-Foster and Edward C. Holmes and Thomas Briese and W. Ian Lipkin},
  journal={Journal of Virology},
  pages={6209 - 6217}
ABSTRACT Israel acute paralysis virus (IAPV) is associated with colony collapse disorder of honey bees. Nonetheless, its role in the pathogenesis of the disorder and its geographic distribution are unclear. Here, we report phylogenetic analysis of IAPV obtained from bees in the United States, Canada, Australia, and Israel and the establishment of diagnostic real-time PCR assays for IAPV detection. Our data indicate the existence of at least three distinct IAPV lineages, two of them circulating… 

First report of Israeli acute paralysis virus in asymptomatic hives of Argentina.

The preliminary results of a survey carried out to detect IAPV in samples taken from several Argentine provinces are reported, by using a reverse transcription Polymerase Chain Reaction assay, and indicate the existence of high frequency of I APV in asymptomatic hives of Argentina.

Short communication: first detection of Israeli Acute Paralysis Virus (IAPV) in Spanish honeybees

Spanish bee samples were analyzed for the presence of Israel Acute Paralysis Virus and no causal relation between IAPV and CCD in Spain was found.

A potentially novel overlapping gene in the genomes of Israeli acute paralysis virus and its relatives

A recently developed method for the estimation of selection in overlapping genes to detect purifying selection and, hence, functionality is applied to provide evolutionary evidence for the existence of a functional overlapping gene, which is translated in the +1 reading frame of the structural polyprotein gene.

Short communication. First detection of Israeli Acute Paralysis Virus (IAPV) in Spanish honeybees

No causal relation between IAPV and CCD in Spain was found and the Spanish isolate was mainly related with the American ones.

A Reverse Genetics System for the Israeli Acute Paralysis Virus and Chronic Bee Paralysis Virus

Developing infectious clones of IAPV and CBPV using a universal vector will allow further dissection of the role of each gene in the context of viral infection while helping to study viral pathogenesis and develop antiviral drugs using reverse genetics systems.

Dynamics of the Presence of Israeli Acute Paralysis Virus in Honey Bee Colonies with Colony Collapse Disorder

It is found that IAPV was the dominant pathogen and it replicated actively in the colonies: viral titers decreased from April to September and increased from September to December, indicating that once acquired and induced to replication it acts as an infectious factor that affects the health of the colonies and may determine their survival.

Israeli Acute Paralysis Virus: Epidemiology, Pathogenesis and Implications for Honey Bee Health

This study shows that IAPV is established as a persistent infection in honey bee populations, likely enabled by both horizontal and vertical transmission pathways, and indicates a novel therapeutic strategy for limiting multiple honey bee viruses simultaneously and reducing colony losses due to viral diseases.

Metagenomic Detection of Viral Pathogens in Spanish Honeybees: Co-Infection by Aphid Lethal Paralysis, Israel Acute Paralysis and Lake Sinai Viruses

The detection of three viruses, Aphid Lethal Paralysis Virus (ALPV), Israel Acute Paralysis virus (IAPV), and Lake Sinai Virus (LSV), in honeybees from Spain is reported, the first identification of such a strain in Europe.

Assembly of Recombinant Israeli Acute Paralysis Virus Capsids

It is shown that coupling the IAPV major structural precursor protein ORF2 to its cognate 3C-like processing enzyme results in processing of the precursor to the individual structural proteins in a number of insect cell lines following expression by a recombinant baculovirus.

Short communication. Presence, quantification and phylogeny of Israeli acute paralysis virus of honeybees in Andalusia (Spain)

Phylogenetic analysis revealed that Andalusian isolates belong to a different lineage to a previously described isolate found in Valencia (2010), which will help to understand the epidemiology and effect of IAPV on Spanish colonies.



Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination.

Sequence analysis indicated that IAPV (Israeli acute paralysis virus) is a distinct dicistrovirus, most homologous to Kashmir bee virus and acute bee paralysis virus.

Phylogenetic evidence for recombination in dengue virus.

The breakpoints were found to be in similar positions, within the fusion peptide of the envelope protein, demonstrating that a single recombination event occurred prior to the divergence of these two strains, the first report of recombination in natural populations of dengue virus.

Characterisation and serological relationships of strains of Kashmir bee virus

Immunodiffusion tests and Western blotting studies indicated that the five virus isolates were serologically related and all were related to acute paralysis virus (APV), the world distribution of KBV strains and their apparent relationship with APV are discussed.

Genetic evidence for coinfection of honey bees by acute bee paralysis and Kashmir bee viruses.

  • J. Evans
  • Biology
    Journal of invertebrate pathology
  • 2001
Nucleotide sequence analyses were used to identify acute bee paralysis virus (ABPV) and Kashmir bee virus (KBV) isolated from a single honey bee colony, finding a coexistence not yet seen between these two viruses.

Molecular diagnosis of Kashmir bee virus infection

Molecular protocols were applied to the diagnosis of Kashmir bee virus infection in the honey bee, Apis mellifera, and procedures based on either serology or nucleic acid amplification proved to be very effective, although it was possible in some circumstances to detect viral infection simply by Coomassie blue staining of SDS-PAGE gels.

Molecular phylogenetics and the classification of honey bee viruses

The phylogenetic relationships of several picorna-like RNA viruses found in honey bees are presented, with respect to 13 additional plant and animal positive-strand RNA viruses, suggesting that sequence-based methods for bee virus identification must be tailored to at least three different virus clades independently.

Recombination in Circulating Enteroviruses

It is concluded that a majority, if not all, circulating enterovirus B strains are recombinants relative to the prototype strains, isolated mostly in the 1950s, and a novel model ofEnterovirus genetics is proposed: the enterov virus genome is a stable symbiosis of genes, and enteroviral species consist of a finite set of capsid genes responsible for different serotypes and a continuum of nonstructural protein genes that seem to evolve in a relatively independent manner.

Egypt Bee Virus and Australian Isolates of Kashmir Bee Virus

Summary A virus unrelated to any other from bees was isolated from diseased adults of Apis mellifera from Egypt. It has isometric particles about 30 nm in diam., which contain RNA and sediment at

Editorial: Molecular epidemiology of dengue virus – the time for big science

  • E. Holmes
  • Medicine
    Tropical medicine & international health : TM & IH
  • 1998
The purpose here is not to review the available data on strain–disease associations, which is ambiguous at best, but to propose areas in which these studies might be improved, and whether the genetic variation the authors see in dengue virus is of clinical importance.

Multiple virus infections in the honey bee and genome divergence of honey bee viruses.