Genetic, cytogenetic, and molecular analyses of mutations induced by melphalan demonstrate high frequencies of heritable deletions and other rearrangements from exposure of postspermatogonial stages of the mouse.

@article{Russell1992GeneticCA,
  title={Genetic, cytogenetic, and molecular analyses of mutations induced by melphalan demonstrate high frequencies of heritable deletions and other rearrangements from exposure of postspermatogonial stages of the mouse.},
  author={Liane Brauch Russell and Patricia R. Hunsicker and Nestor L. A. Cacheiro and Eugene M. Rinchik},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 13},
  pages={6182-6}
}
Specific-locus experiments have previously shown melphalan to be mutagenic in all male germ-cell stages tested and particularly so in early spermatids. All but 2 of 24 specific-locus mutations recovered were tested genetically, cytogenetically, and/or molecularly. At least 12 of 15 tested mutations recovered from postspermatogonial stages but only 1 of 7 mutations recovered from stem-cell or differentiating spermatogonia gave evidence of being deletions or other rearrangements. Melphalan… CONTINUE READING