Genes of the thymidine salvage pathway: thymine-7-hydroxylase from a Rhodotorula glutinis cDNA library and iso-orotate decarboxylase from Neurospora crassa.

@article{Smiley2005GenesOT,
  title={Genes of the thymidine salvage pathway: thymine-7-hydroxylase from a Rhodotorula glutinis cDNA library and iso-orotate decarboxylase from Neurospora crassa.},
  author={Jeffrey A Smiley and Melisa L Kundracik and Daniel A Landfried and Vincient R Barnes and Armend A Axhemi},
  journal={Biochimica et biophysica acta},
  year={2005},
  volume={1723 1-3},
  pages={256-64}
}
Genes for two enzymes in the thymidine salvage pathway, thymine-7-hydroxylase (THase; official name thymine dioxygenase) and iso-orotate decarboxylase (IDCase) have been isolated from fungal sources. THase was isolated from a Rhodotorula glutinis cDNA library using a degenerate oligonucleotide based on the published amino acid sequence. The coding sequence was transferred to an Escherichia coli expression system, from which recombinant THase activity was measured using 14C-labeled thymine. The… CONTINUE READING
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