Genes involved in protein glycosylation determine the activity and cell internalization of the antifungal peptide PAF26 in Saccharomyces cerevisiae.

@article{Harries2013GenesII,
  title={Genes involved in protein glycosylation determine the activity and cell internalization of the antifungal peptide PAF26 in Saccharomyces cerevisiae.},
  author={Eleonora del M. Harries and Lourdes Carmona and Alberto Mu{\~n}oz and Jos{\'e} Ignacio Ibeas and Nick D Read and M{\'o}nica Gand{\'i}a and Jose F Marcos},
  journal={Fungal genetics and biology : FG & B},
  year={2013},
  volume={58-59},
  pages={105-15}
}
We have previously characterized the synthetic hexapeptide PAF26 as a cell-penetrating and non-lytic antifungal peptide that is active against Saccharomyces cerevisiae and filamentous fungi. Numerous cell wall (CW) proteins are glycosylated in fungi and many of these play important roles in fungal pathogenesis. In this study, we screened a collection of S. cerevisiae deletion mutants for protein glycosylation genes whose deletion altered the sensitivity to PAF26. Increased tolerance to PAF26… CONTINUE READING