Genes encoding ribonucleoside hydrolase 1 and 2 from Corynebacterium ammoniagenes.

@article{Kim2006GenesER,
  title={Genes encoding ribonucleoside hydrolase 1 and 2 from Corynebacterium ammoniagenes.},
  author={Hyun-Soo Kim and Jin-Ho Lee and W S Lee and W S Bang},
  journal={Microbiology},
  year={2006},
  volume={152 Pt 4},
  pages={1169-77}
}
Two kinds of nucleoside hydrolases (NHs) encoded by rih1 and rih2 were cloned from Corynebacterium ammoniagenes using deoD- and gsk-defective Escherichia coli. Sequence analysis revealed that NH 1 was a protein of 337 aa with a deduced molecular mass of 35,892 Da, whereas NH 2 consisted of 308 aa with a calculated molecular mass of 32 310 Da. Experiments with crude extracts of IPTG-induced E. coli CGSC 6885(pTNU23) and 6885(pTNI12) indicated that the Rih1 enzyme could catalyse the hydrolysis of… CONTINUE READING

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