Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates

@article{Torres2009GenesRT,
  title={Genes Related to Long Polar Fimbriae of Pathogenic Escherichia coli Strains as Reliable Markers To Identify Virulent Isolates},
  author={Alfredo G. Torres and M. Blanco and P. Valenzuela and T. M. Slater and Shilpa D. Patel and G. Dahbi and C. López and Ximena Fernández Barriga and Jesús E. Blanco and T. A. Gomes and R. Vidal and Jorge Blanco},
  journal={Journal of Clinical Microbiology},
  year={2009},
  volume={47},
  pages={2442 - 2451}
}
  • Alfredo G. Torres, M. Blanco, +9 authors Jorge Blanco
  • Published 2009
  • Medicine, Biology
  • Journal of Clinical Microbiology
ABSTRACT Lpf (stands for long polar fimbriae) is one of the few adhesive factors of enterohemorrhagic Escherichia coli O157:H7 associated with colonization of the intestine. E. coli O157:H7 strains possess two lpf loci encoding highly regulated fimbrial structures. Database analysis of the genes encoding the major fimbrial subunits demonstrated that they are present in commensal as well as pathogenic (both intestinal and extraintestinal) E. coli strains and in Salmonella strains and that the… Expand
Identification of the long polar fimbriae gene variants in the locus of enterocyte effacement-negative Shiga toxin-producing Escherichia coli strains isolated from humans and cattle in Argentina.
TLDR
The long polar fimbriae (Lpf) is one of few adhesive factors of Shiga toxin-producing Escherichia coli (STEC) and it is associated with colonization of the intestine and the association of some of these isolates carrying specific lpf-variants with disease is defined. Expand
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TLDR
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In the in vitro and in vivo models, the presence of the wild type O104:H4 facilitates increased adherence of the ΔlpfA1 to levels exceeding that of theWild type, and demonstrated that Lpf1 is one of the factors responsible for O104?:H4 intestinal adhesion and colonization. Expand
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Characteristics of the Shiga-toxin-producing enteroaggregative Escherichia coli O104:H4 German outbreak strain and of STEC strains isolated in Spain.
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Cellular and Infection Microbiology
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    References

    SHOWING 1-10 OF 49 REFERENCES
    Characterization of the second long polar (LP) fimbriae of Escherichia coli O157:H7 and distribution of LP fimbriae in other pathogenic E. coli strains.
    TLDR
    The results suggest that LP fimbriae 2 may contribute to the early stages of EHEC adhesion and that genes encoding the majorLP fimbrial subunits are present in a small group of EhEC and EPEC serotypes. Expand
    Contribution of Long Polar Fimbriae to the Virulence of Rabbit-Specific Enteropathogenic Escherichia coli
    TLDR
    It is suggested that lpfR141 contributes to the early stages of REPEC-mediated disease and that this is important for the development of severe diarrhea in susceptible animals. Expand
    The long polar fimbriae genes identified in Shiga toxin-producing Escherichia coli are present in other diarrheagenic E. coli and in the standard E. coli collection of reference (ECOR) strains.
    TLDR
    This study showed that the lpfA genes identified in the genome of STEC strains are not specific to this category and suggested that there is a relationship between the l pfA variant and the phylogenetic group. Expand
    Identification of a Novel Fimbrial Gene Cluster Related to Long Polar Fimbriae in Locus of Enterocyte Effacement-Negative Strains of Enterohemorrhagic Escherichia coli
    TLDR
    Deletion of the putative major fimbrial subunit gene, lpfA, from EHEC O113:H21 resulted in decreased adherence of this strain to epithelial cells, suggesting that l pfO113 may function as an adhesin in LEE-negative isolates of EH EC. Expand
    Prevalence of the lpfO113 gene cluster among Escherichia coli O157 isolates from different sources.
    TLDR
    Results show that lpfAO113 is widely distributed among eae-negative E. coli isolates and thus may represent an important adherence factor in this group of pathogens. Expand
    Identification and Characterization of lpfABCC′DE, a Fimbrial Operon of Enterohemorrhagic Escherichia coli O157:H7
    TLDR
    It is proposed that LP fimbriae participate in the interaction of E. coli O157:H7 with eukaryotic cells by assisting in microcolony formation and was restored when the lpf operon was introduced on a plasmid. Expand
    Distribution of putative adhesins in Shiga toxin-producing Escherichia coli (STEC) strains isolated from different sources in Chile
    TLDR
    The distribution of three putative adhesin genes in 123 Shiga toxin-producing (STEC) strains was determined by PCR and efa1 and lpfAO157 were the most common in humans strains and saa in food strains. Expand
    Long polar fimbriae and tissue tropism in Escherichia coli O157:H7.
    TLDR
    LP fimbrial genes are the first genes to be identified outside the locus of enterocyte effacement pathogenicity island that influence O157:H7 human intestinal tissue tropism. Expand
    Cloning, Expression, and Characterization of Fimbrial Operon F9 from Enterohemorrhagic Escherichia coli O157:H7
    TLDR
    The main F9 subunit gene was deleted in E. coli O157:H7, and the resulting mutant was compared with the wild-type strain for colonization in weaned cattle, indicating that the adhesin is not responsible for the rectal tropism observed but may contribute to colonization at other sites, as demonstrated previously with very young animals. Expand
    Molecular Evolution of the Intimin Gene in O111 Clones of Pathogenic Escherichia coli
    TLDR
    The results suggest that there have been multiple acquisitions of the LEE in the EHEC 2/EPEC 2 clonal lineage, with a recent turnover in either O111:H8 or its close relatives. Expand
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