Genes Involved in Galactooligosaccharide Metabolism in Lactobacillus reuteri and Their Ecological Role in the Gastrointestinal Tract

  title={Genes Involved in Galactooligosaccharide Metabolism in Lactobacillus reuteri and Their Ecological Role in the Gastrointestinal Tract},
  author={Monchaya Rattanaprasert and Jan-Peter van Pijkeren and Amanda E. Ramer-Tait and Maria Quintero and Car Reen Kok and Jens Walter and Robert Hutkins},
  journal={Applied and Environmental Microbiology},
This study shows that lac genes in Lactobacillus reuteri encode hydrolases and transporters that are necessary for the metabolism of GOS, as well as α-galactoside substrates. Coculture experiments with the wild-type strain and a gos mutant clearly demonstrated that GOS utilization confers a growth advantage in medium containing GOS as the sole carbohydrate source. However, the wild-type strain also outcompeted the mutant in germfree mice, suggesting that GOS genes in L. reuteri also provide a… 
Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum
The results enable the design of highly-selective synbiotic combinations of L. plantarum strain-specific probiotics and specific GOS-prebiotic fractions and confirm the role of these genes in the import and utilization of higher-DP GOS constituents.
A Phylogenetic View on the Role of Glycerol for Growth Enhancement and Reuterin Formation in Limosilactobacillus reuteri
Differences in the substrate-specific regulation of the pdu cluster in L. reuteri lineages are suggested that might be reflective of their ecological niches, e.g., chicken foregut versus human infant and adult large intestine.
Comparative Genomic and Functional Evaluations of Bacillus subtilis Newly Isolated from Korean Traditional Fermented Foods
The findings suggest that the newly isolated B. subtilis strains can be a good candidate for probiotic with further in-depth investigation on health benefits and safety.
Characteristic analysis of the fermentation and sporulation properties of the traditional sake yeast strain Hiroshima no.6
It is presented that the fermentation ability of H 6 is suitable for sake brewing, and the precursor of dimethyl trisulfide in sake from H6 is low, and phylogenetic analysis indicated that H7 is closely related to the K7 group.
Lactobacillus reuteri strains protect epithelial barrier integrity of IPEC‐J2 monolayers from the detrimental effect of enterotoxigenic Escherichia coli
It is demonstrated that L. reuteri strains exert a protective effect against ETEC‐induced mucosal integrity disruption, as monitored by permeability of FITC‐dextran and transepithelial electrical resistance (TEER).
Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption
Despite the prebiotic’s substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.
Characteristics and expression patterns of six α-galactosidases in cucumber (Cucumis sativus L.).
Phylogenetic analysis of collected α-Gal amino acid sequences from different plants indicated that the ancestor of both acid and alkaline α-Gals existed before monocots and dicots separated.


Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus
P phenotypic confirmation of the functional LacS permease not only for the utilization of lactose and GOS but also lactitol is confirmed, suggesting a prominent role of LacS in the metabolism of a broad range of prebiotic β-galactosides, known to selectively modulate the beneficial gut microbiota.
Genetic and phenotypic analysis of carbohydrate metabolism and transport in Lactobacillus reuteri.
Characterization of the melA Locus for α-Galactosidase in Lactobacillus plantarum
This first genetic characterization of melA and of its putative associated transporter, rafP, in a lactobacillus opens doors to various applications both in the manufacture of soy-derived products and in probiotic and nutraceutical issues.
Bifidobacterium carbohydrases-their role in breakdown and synthesis of (potential) prebiotics.
A review of the possibilities to produce new classes of non-digestible oligosaccharides by showing the presence and (transglycosylation) activity of the most important carbohydrate modifying enzymes in bifidobacteria.
In vitro fermentation of lactulose by human gut bacteria.
More attention should be paid to bacteria besides bifidobacteria and lactobacilli to further investigate the relationship between functional oligosaccharides and gut bacteria, as well as to identify lactulose-metabolizing bacteria in the human gut, according to predictions.
Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM
Differential transcriptomics and functional genomics were used to identify genes in Lactobacillus acidophilus NCFM involved in the uptake and catabolism of 11 potential prebiotic compounds consisting of α- and β- linked galactosides and glucosides, highlighting the broad oligosaccharide metabolic repertoire of L. acidophillus NCFM.
Prebiotic Galactooligosaccharide Metabolism by Probiotic Lactobacilli and Bifidobacteria.
In this study, commercially available bifidobacteria and lactobacilli probiotic strains were evaluated for growth in the presence of GOS and Lactobacillus acidophilus NCFM (LA_NCFM) was found to efficiently consume all GOS oligomers.
Ecological Role of Lactobacilli in the Gastrointestinal Tract: Implications for Fundamental and Biomedical Research
  • J. Walter
  • Biology, Medicine
    Applied and Environmental Microbiology
  • 2008
Evidence is summarized that suggests that only a small number of Lactobacillus species are true inhabitants of the mammalian intestinal tract and that most lactobacilli present are allochthonous members derived from fermented food, the oral cavity, or more proximal parts of the GIT.