Generation time of Candida albicans in synchronized and nonsynchronized cultures

  title={Generation time of Candida albicans in synchronized and nonsynchronized cultures},
  author={N. Dabrowa and J. Landau and V. Newcomer},
  journal={Medical Mycology},
Synchronized growth of Candida albicans was induced. The generation time of the synchronized cells was compared with those of nonsynchronized cells cultured in Sabouraud broth, Medium 199, and a synthetic synchronization medium. The mean generation time of the synchronized cultures was 3·2 hours while that of the nonsynchronized cultures ranged from 1·7 to 3·6 hours. The generation time in shake cultures of nonsynchronized cells was uniformly shorter than that of the corresponding aerated… Expand
Growth and form in Candida albicans.
It is concluded that in human serum or plasma, although the growth of C. albicans is not inhibited, a transient cessation of multiplication occurs and possible reasons for this are discussed. Expand
A Comparison of Germ Tube Production by Candida albicans in Three Media
Medium 199 and Glucose-Glycine Medium at 37˚C were highly satisfactory and gave 90 % conversion of yeast cells to hyphal-form cells and appeared to give more rapid germ tube production than 199 Medium. Expand
Synthesis of nueic acids and proteins in the dimorphic forms of Candida albicans.
Electrophoretic analysis of the water soluble proteins from budding and filament producing cells revealed that C. albicans grows in tissue culture medium 199 buds when incubated at 25°C but produces filaments when incubation at 37°C. Expand
Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain, or temperature
The extent of change in cytoplasmic proteins which accompanies yeast-to-mycelium morphogenesis of Candida albicans was analyzed by two-dimensional gel electrophoresis and suggests that proteins unique to either phase may serve a regulatory function. Expand
Effects of pH and temperature on the in vitro growth of Sporothrix schenckii.
Five strains of Sporothrix schenckii were grown in liquid shake cultures with an extended lag phase and a reduced total growth when incubated at 39°C or 41°C and pH of 5·6, 7·0 and 7·4. Expand
Factors affecting filamentation in Candida albicans: relationship of the uptake and distribution of proline to morphogenesis
Filamentation under conditions of low phosphate, high glucose, and increased cellular reduction potential, accompanied by a decrease in Kreb's cycle activity, suggests that morphogenesis in C. albicans is correlated with a Crabtree-like effect, i.e., repression of mitochondrial activity. Expand
In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation
  • N. Bhattarai, K. Rai, S. Rijal, B. Khanal
  • Biology, Medicine
  • The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale
  • 2017
Growth inhibition of Leishmania donovani is significantly lower with caspofungin and could be used to protect the parasite cultivation from fungal contamination. Expand
The Candida albicans Hsp90 co-chaperone complex regulates aspects of virulence
• Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for anyExpand
Mykologisehe Befunde bei Gastroskopiepatienten — Möglichkeiten einer differenzierten Bewertung/Mycological Results Obtained by Gastroscopy — Aspects for Evaluation
Zusammenfassung: Bei 44 von 77 unserer Patienten (57,1%) war der während einer Gastroskopie gewonnene Magensaft hefe‐positiv, wodurch es möglich war, in 64,9% eine eindeutige Bewertung vorzunehmen. Expand


The blastospores of Candida albicans form filaments or “germ tubes” within two to three hours when incubated at 37° C in the presence of serum or plasma derived from humans and animals, and little information is available concerning the particular property or properties of serum responsible for the formation of filaments. Expand
The behaviour of nucleic acids in synchronously dividing cultures of Saccharomyces cerevisiae.
When inoculated into a synthetic medium, cells of Saccharomyces cerevisiae which had previously been subjected to repeated suspension alternately in two different media, were found to divide with a marked degree of synchrony and buds were formed almost immediately after cleavage. Expand
Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.∗,†
Although this mixture will not support cell life indefinitely, it has been found adequate to maintain chick embryo cells for an average period of 4 to 5 weeks. Expand
Timing of enzyme synthesis during synchronous division in yeast.
Taxonomy of Yeasts