Generation of two polyclonal antibodies for western blot detection of hepatitis A virus in plants

Abstract

In recombinant systems, the availability of good quality antibodies is required to detect Hepatitis A virus (HAV) proteins. Following this line, the cDNA sequences coding for two hepatitis A virus structural proteins, VP2 and VP3, were cloned into the bacterial expression vector pTrcHis yielding plasmids pTrcVP2 and pTrcVP3. Recombinant Escherichia coli XL1… (More)

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