Generation of tumor cell lysate-loaded dendritic cells preprogrammed for IL-12 production and augmented T cell response

@article{Vgh2003GenerationOT,
  title={Generation of tumor cell lysate-loaded dendritic cells preprogrammed for IL-12 production and augmented T cell response},
  author={Zsuzsa V{\'e}gh and Amitabha Mazumder},
  journal={Cancer Immunology, Immunotherapy},
  year={2003},
  volume={52},
  pages={67-79}
}
Dendritic cells (DC) loaded with tumor associated antigens (TAA) are often used for the vaccination of cancer patients; however methodologies for the vaccine preparation have not yet been standardized. The purpose of this work was to optimize the ex-vivo production of functional TAA-loaded DC that would produce interleukin-2 (IL-12) and enhance the T cell response. We generated ex-vivo DC from human monocytes with granulocyte macrophage-colony stimulating factor (GM-CSF) and IL-4, and whole… CONTINUE READING