Generation of transgenic porcine chimeras using primordial germ cell-derived colonies.

@article{Piedrahita1998GenerationOT,
  title={Generation of transgenic porcine chimeras using primordial germ cell-derived colonies.},
  author={Jorge A Piedrahita and Karen L. Moore and Betty K Oetama and Chang-Kyu Lee and Nicholas Scales and Jagdeece Ramsoondar and Fuller W Bazer and Thomas Ott},
  journal={Biology of reproduction},
  year={1998},
  volume={58 5},
  pages={1321-9}
}
In mice, two pluripotent cell lines, embryonic stem (ES) cells and embryonic germ (EG) cells, have been identified. We present here results indicating that porcine EG cell lines can be isolated, genetically transformed, and utilized to make transgenic chimeras. Briefly, primordial germ cells (PGCs) were isolated from Day 25-27 fetuses and plated on STO feeder cells in Dulbecco's modified Eagle's medium:Ham's F-10 medium supplemented with 0.01 mM nonessential amino acids, 2 mM glutamine, 15… CONTINUE READING