Generation of transgene-free human induced pluripotent stem cells with an excisable single polycistronic vector

@article{Papapetrou2011GenerationOT,
  title={Generation of transgene-free human induced pluripotent stem cells with an excisable single polycistronic vector},
  author={Eirini P Papapetrou and Michel Sadelain},
  journal={Nature Protocols},
  year={2011},
  volume={6},
  pages={1251-1273}
}
The generation of induced pluripotent stem cells (iPSCs) devoid of permanently integrated reprogramming factor genes is essential to reduce differentiation biases and artifactual phenotypes. We describe a protocol for the generation of human iPSCs using a single polycistronic lentiviral vector (pLM-fSV2A) coexpressing OCT4, SOX2, KLF4 and c-MYC; this is flanked by two loxP sites in its long terminal repeats (LTRs). Human iPSC lines are established with an efficiency of up to 1% and screened to… CONTINUE READING
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