Generation of tetracycline-inducible conditional gene knockout cells in a human Nalm-6 cell line.

Abstract

Conditional gene knockout by homologous recombination combined with an inducible gene expression system is a powerful approach for studying gene function, although homologous recombination in human cells occurs infrequently. The tetracycline-regulated gene expression (Tet-Off) system is a convenient method for achieving conditional gene knockout, but it is… (More)
DOI: 10.1016/j.jbiotec.2008.12.010

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