Generation of superoxide anion by succinate-cytochrome c reductase from bovine heart mitochondria.

@article{Zhang1998GenerationOS,
  title={Generation of superoxide anion by succinate-cytochrome c reductase from bovine heart mitochondria.},
  author={Li Zhang and Linda Yu and C. A. Yu},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 51},
  pages={
          33972-6
        }
}
Production of superoxide anion (O-2), measured as the chemiluminescence of the 2-methyl-6-(p-methoxyphenyl)-3, 7-dihydroimidazo[1,2-a]pyrazin-3-one hydrochloride (MCLA)-O-2 adduct, was observed during electron transfer from succinate to cytochrome c by reconstituted succinate-cytochrome c reductase-phospholipid vesicles replenished with succinate dehydrogenase. Addition of carbonyl cyanide p-trifluoromethoxyphenylhydrazone or detergent to the reconstituted reductase-phospholipid vesicles… CONTINUE READING

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