Generation of retinal cells from mouse and human induced pluripotent stem cells

@article{Hirami2009GenerationOR,
  title={Generation of retinal cells from mouse and human induced pluripotent stem cells},
  author={Yasuhiko Hirami and Fumitaka Osakada and Kazutoshi Takahashi and Keisuke Okita and Shinya Yamanaka and Hanako Ohashi Ikeda and Nagahisa Yoshimura and Masayo Takahashi},
  journal={Neuroscience Letters},
  year={2009},
  volume={458},
  pages={126-131}
}

Generation, Purification and Transplantation of Photoreceptors Derived from Human Induced Pluripotent Stem Cells

Evidence is provided that enriched populations of human photoreceptors can be derived from iPS cells, and it is found that when the FACS purified iPSC derived photoreCEPTs are transplanted into a normal mouse retina and express photoreceptor markers.

Induced Pluripotent Stem Cells: Development in the Ophthalmologic Field

Human induced pluripotent stem cells (iPSCs) are a type of stem cells that can be derived from human somatic cells by introducing certain transcription factors, which make them viable for transplantation and individual disease modeling.

Derivation of Functional Retinal Pigmented Epithelium from Induced Pluripotent Stem Cells

iPSCs generated using Oct4, Sox2, Nanog, and Lin28 can spontaneously differentiate into RPE cells, which can then be isolated and cultured to form highly differentiated RPE monolayers, and further supports the finding that iPSCs are similar to hESCs in their differentiation potential.

Defining the Integration Capacity of Embryonic Stem Cell‐Derived Photoreceptor Precursors

It is concluded that exclusion of proliferative cells from ESC‐derived cultures is essential for effective transplantation and despite showing expression profiles characteristic of immature photoreceptors, the ESC‐ derived precursors generated using this protocol did not display transplantation competence equivalent to precursor from the postnatal retina.

Advances in Retinal Stem Cell Biology

  • A. Viczian
  • Biology
    Journal of ophthalmic & vision research
  • 2013
This review focuses on the culture methods used by different groups, which has culminated in the generation of laminated retinal tissue from both embryonic and induced pluripotent cells.

Efficient Stage‐Specific Differentiation of Human Pluripotent Stem Cells Toward Retinal Photoreceptor Cells

The data highlight an important role for Dkk1 and Noggin in enhancing the differentiation of hESC and hiPSC toward retinal progenitor cells and photoreceptor precursors during the early stages of differentiation, while suggesting that further maturation of these cells into photoreceptors may not require additional factors and can ensue under minimal culture conditions.

In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction

Both three-factor and four-factor human iPS cells could be successfully differentiated into retinal cells by small-molecule induction, providing a solution to the problem of cross-species antigenic contamination in cell-replacement therapy, and is also useful for in vitro modeling of development, disease and drug screening.

Hypoxia enhances the generation of retinal progenitor cells from human induced pluripotent and embryonic stem cells.

Mimicking physiological O(2) tension is a favorable condition for the efficient generation of RPCs from both hiPSCs and hESCs, indicating that efficient differentiation of retinal cells from human pluripotent stem cells remains a major challenge.

Derivation, characterization and retinal differentiation of induced pluripotent stem cells

The derivation of four iPSC lines from mouse embryonic fibroblasts (MEFs) using a cocktail of recombinant retroviruses carrying the genes for Oct4, Sox2, Klf4 and cMyc is reported, showing a great promise for generating different cell types of the retina.
...

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