Generation of retinal cells from mouse and human induced pluripotent stem cells

  title={Generation of retinal cells from mouse and human induced pluripotent stem cells},
  author={Yasuhiko Hirami and Fumitaka Osakada and Kazutoshi Takahashi and Keisuke Okita and Shinya Yamanaka and Hanako Ohashi Ikeda and Nagahisa Yoshimura and Masayo Takahashi},
  journal={Neuroscience Letters},

Generation, Purification and Transplantation of Photoreceptors Derived from Human Induced Pluripotent Stem Cells

Evidence is provided that enriched populations of human photoreceptors can be derived from iPS cells, and it is found that when the FACS purified iPSC derived photoreCEPTs are transplanted into a normal mouse retina and express photoreceptor markers.

Induced Pluripotent Stem Cells: Development in the Ophthalmologic Field

Human induced pluripotent stem cells (iPSCs) are a type of stem cells that can be derived from human somatic cells by introducing certain transcription factors, which make them viable for transplantation and individual disease modeling.

Defining the Integration Capacity of Embryonic Stem Cell‐Derived Photoreceptor Precursors

It is concluded that exclusion of proliferative cells from ESC‐derived cultures is essential for effective transplantation and despite showing expression profiles characteristic of immature photoreceptors, the ESC‐ derived precursors generated using this protocol did not display transplantation competence equivalent to precursor from the postnatal retina.

Advances in Retinal Stem Cell Biology

  • A. Viczian
  • Biology
    Journal of ophthalmic & vision research
  • 2013
This review focuses on the culture methods used by different groups, which has culminated in the generation of laminated retinal tissue from both embryonic and induced pluripotent cells.

Efficient Stage‐Specific Differentiation of Human Pluripotent Stem Cells Toward Retinal Photoreceptor Cells

The data highlight an important role for Dkk1 and Noggin in enhancing the differentiation of hESC and hiPSC toward retinal progenitor cells and photoreceptor precursors during the early stages of differentiation, while suggesting that further maturation of these cells into photoreceptors may not require additional factors and can ensue under minimal culture conditions.

In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction

Both three-factor and four-factor human iPS cells could be successfully differentiated into retinal cells by small-molecule induction, providing a solution to the problem of cross-species antigenic contamination in cell-replacement therapy, and is also useful for in vitro modeling of development, disease and drug screening.

Hypoxia enhances the generation of retinal progenitor cells from human induced pluripotent and embryonic stem cells.

Mimicking physiological O(2) tension is a favorable condition for the efficient generation of RPCs from both hiPSCs and hESCs, indicating that efficient differentiation of retinal cells from human pluripotent stem cells remains a major challenge.

Differentiation of human pluripotent stem cells to retinal pigmented epithelium in defined conditions using purified extracellular matrix proteins

Identification of key ECM proteins may assist with future scaffold designs and provide peptide sequences for use in synthetic, xeno‐free, GMP‐compliant generation of RPE from human pluripotent stem cells relevant to clinical translation.

Generation of retinal ganglion-like cells from reprogrammed mouse fibroblasts.

The findings demonstrate that iPS cells are valuable for regeneration research into retinal degeneration diseases and could be further turned into RG-like cells by the regulation of transcription factor expression.



Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells

In vitro generation of putative rod and cone photoreceptors from mouse, monkey and human ES cells by stepwise treatments under defined culture conditions, in the absence of retinal tissues, may facilitate the development of human ES cell–based transplantation therapies for retinal diseases.

Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells.

Direct differentiaion of retinal precursors in vitro from mouse ES cells generated with selective integration and survival of ES cell-derived cells with the photoreceptor phenotype (marker expression and morphology) in the outer nuclear layer of the retina.

Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts

A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.

Fluorescence‐Activated Cell Sorting–Based Purification of Embryonic Stem Cell–Derived Neural Precursors Averts Tumor Formation after Transplantation

Results indicate that Sox1‐based cell sorting of neural precursors prevents graft‐derived tumor formation after transplantation, providing a promising strategy for cell transplantation therapy of neurodegenerative disorders.

Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation

The use of episomal expression technology for functional evaluation of cDNA clones during ES-cell differentiation in vitro revealed that the Wnt antagonist Sfrp2 stimulates production of neural progenitors and reveals the importance of Wnt signaling in regulating ES- cell lineage diversification.

Directed differentiation of telencephalic precursors from embryonic stem cells

In the absence of caudalizing signals, floating aggregates of ES cells generate naive telencephalic precursors that acquire subregional identities by responding to extracellular patterning signals.

Soluble factors and the development of rod photoreceptors

Recent progress toward understanding how environmental factors influence the development of vertebrate rod photoreceptors is described.