Generation of recombinant lentogenic Newcastle disease virus from cDNA.

  title={Generation of recombinant lentogenic Newcastle disease virus from cDNA.},
  author={Angela Roemer-Oberdoerfer and Egbert Mundt and Teshome Mebatsion and Ursula J. Buchholz and Thomas C Mettenleiter},
  journal={The Journal of general virology},
  volume={80 ( Pt 11)},
Recombinant lentogenic Newcastle disease virus (NDV) of the vaccine strain Clone-30 was reproducibly generated after simultaneous expression of antigenome-sense NDV RNA and NDV nucleoprotein, phosphoprotein and RNA-dependent RNA polymerase from plasmids transfected into cells stably expressing T7 RNA polymerase. For this purpose, the genome of Clone-30, comprising 15186 nt, was cloned and sequenced prior to assembly into a full-length cDNA clone under control of a T7 RNA polymerase promoter… CONTINUE READING